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Journal: Non-coding RNA Research
Article Title: Exosomal miRNA-218–5p derived from low-passage dermal papilla cells modulates hair follicle growth and development
doi: 10.1016/j.ncrna.2026.01.004
Figure Lengend Snippet: DPC-Exos transported miR-218-5p from DPCs to HFSCs. (A) Fluorescence imaging of HFSCs after treatment with DiI-labeled DPC-Exos (scale bar = 50 μm). (B) Schematic of the DPC–HFSC co-culture system with fluorescence showing intercellular transfer of exosomal miR-218–5p. (C) MiR-218–5p expression in HFSCs after transfection with Cy3-miR-218–5p (unpaired two-tailed t -test, n = 3). (D) MiR-218–5p expression in HFSCs after DPCs were transfected with siRNA-Drosha (unpaired two-tailed t -test, n = 3). (E) MiR-218–5p expression in HFSCs after DPCs were treated with GW4869 (unpaired two-tailed t -test, n = 3). ∗∗ P < 0.01.
Article Snippet: After three times washing with PBS containing 0.1 % Tween-20 (Solarbio, China, Cat. No. ST825),
Techniques: Fluorescence, Imaging, Labeling, Co-Culture Assay, Expressing, Transfection, Two Tailed Test
Journal: Neurology® Neuroimmunology & Neuroinflammation
Article Title: Low Prevalence and Inconsistency of LRP4-IgG Detection in Suspected Myasthenia Gravis
doi: 10.1212/NXI.0000000000200554
Figure Lengend Snippet: Three protocols of CBA were performed: l-CBA (A–C), pf-CBA (D–F), and mf-CBA (G–I). 4′,6-diamidino-2-phenylindole (DAPI) (blue channel) shows nuclear staining, and green fluorescent protein (GFP) expression (green channel) indicates transfected cells. For each protocol, LRP4 antibody was visualized with Cy3 anti-mouse IgG (red fluorescence). CBA = cell-based assay; LRP4-IgG = lipoprotein receptor-related protein 4; mf-CBA = methanol-fixed CBA; pf-CBA = paraformaldehyde-fixed CBA.
Article Snippet: For its detection,
Techniques: Staining, Expressing, Transfection, Fluorescence, Cell Based Assay