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Proteintech secondary antibodies cy3 conjugated goat anti mouse lgg
DPC-Exos transported miR-218-5p from DPCs to HFSCs. (A) Fluorescence imaging of HFSCs after treatment with DiI-labeled DPC-Exos (scale bar = 50 μm). (B) Schematic of the DPC–HFSC co-culture system with fluorescence showing intercellular transfer of exosomal miR-218–5p. (C) MiR-218–5p expression in HFSCs after transfection with <t>Cy3-miR-218–5p</t> (unpaired two-tailed t -test, n = 3). (D) MiR-218–5p expression in HFSCs after DPCs were transfected with siRNA-Drosha (unpaired two-tailed t -test, n = 3). (E) MiR-218–5p expression in HFSCs after DPCs were treated with GW4869 (unpaired two-tailed t -test, n = 3). ∗∗ P < 0.01.
Secondary Antibodies Cy3 Conjugated Goat Anti Mouse Lgg, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Jackson Immuno donkey anti guinea pig cy3
DPC-Exos transported miR-218-5p from DPCs to HFSCs. (A) Fluorescence imaging of HFSCs after treatment with DiI-labeled DPC-Exos (scale bar = 50 μm). (B) Schematic of the DPC–HFSC co-culture system with fluorescence showing intercellular transfer of exosomal miR-218–5p. (C) MiR-218–5p expression in HFSCs after transfection with <t>Cy3-miR-218–5p</t> (unpaired two-tailed t -test, n = 3). (D) MiR-218–5p expression in HFSCs after DPCs were transfected with siRNA-Drosha (unpaired two-tailed t -test, n = 3). (E) MiR-218–5p expression in HFSCs after DPCs were treated with GW4869 (unpaired two-tailed t -test, n = 3). ∗∗ P < 0.01.
Donkey Anti Guinea Pig Cy3, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Jackson Immuno donkey anti chicken cy3
DPC-Exos transported miR-218-5p from DPCs to HFSCs. (A) Fluorescence imaging of HFSCs after treatment with DiI-labeled DPC-Exos (scale bar = 50 μm). (B) Schematic of the DPC–HFSC co-culture system with fluorescence showing intercellular transfer of exosomal miR-218–5p. (C) MiR-218–5p expression in HFSCs after transfection with <t>Cy3-miR-218–5p</t> (unpaired two-tailed t -test, n = 3). (D) MiR-218–5p expression in HFSCs after DPCs were transfected with siRNA-Drosha (unpaired two-tailed t -test, n = 3). (E) MiR-218–5p expression in HFSCs after DPCs were treated with GW4869 (unpaired two-tailed t -test, n = 3). ∗∗ P < 0.01.
Donkey Anti Chicken Cy3, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Jackson Immuno goat anti mouse igg antibody conjugated to cy3
DPC-Exos transported miR-218-5p from DPCs to HFSCs. (A) Fluorescence imaging of HFSCs after treatment with DiI-labeled DPC-Exos (scale bar = 50 μm). (B) Schematic of the DPC–HFSC co-culture system with fluorescence showing intercellular transfer of exosomal miR-218–5p. (C) MiR-218–5p expression in HFSCs after transfection with <t>Cy3-miR-218–5p</t> (unpaired two-tailed t -test, n = 3). (D) MiR-218–5p expression in HFSCs after DPCs were transfected with siRNA-Drosha (unpaired two-tailed t -test, n = 3). (E) MiR-218–5p expression in HFSCs after DPCs were treated with GW4869 (unpaired two-tailed t -test, n = 3). ∗∗ P < 0.01.
Goat Anti Mouse Igg Antibody Conjugated To Cy3, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Jackson Immuno cy3 affinipure donkey anti mouse igg
Three protocols of CBA were performed: l-CBA (A–C), pf-CBA (D–F), and mf-CBA (G–I). 4′,6-diamidino-2-phenylindole (DAPI) (blue channel) shows nuclear staining, and green fluorescent protein (GFP) expression (green channel) indicates transfected cells. For each protocol, LRP4 antibody was visualized with <t>Cy3</t> anti-mouse IgG (red fluorescence). CBA = cell-based assay; LRP4-IgG = lipoprotein receptor-related protein 4; mf-CBA = methanol-fixed CBA; pf-CBA = paraformaldehyde-fixed CBA.
Cy3 Affinipure Donkey Anti Mouse Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Jackson Immuno cy3 donkey anti rabbit
Three protocols of CBA were performed: l-CBA (A–C), pf-CBA (D–F), and mf-CBA (G–I). 4′,6-diamidino-2-phenylindole (DAPI) (blue channel) shows nuclear staining, and green fluorescent protein (GFP) expression (green channel) indicates transfected cells. For each protocol, LRP4 antibody was visualized with <t>Cy3</t> anti-mouse IgG (red fluorescence). CBA = cell-based assay; LRP4-IgG = lipoprotein receptor-related protein 4; mf-CBA = methanol-fixed CBA; pf-CBA = paraformaldehyde-fixed CBA.
Cy3 Donkey Anti Rabbit, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Jackson Immuno donkey cy3 conjugated anti rabbit
Three protocols of CBA were performed: l-CBA (A–C), pf-CBA (D–F), and mf-CBA (G–I). 4′,6-diamidino-2-phenylindole (DAPI) (blue channel) shows nuclear staining, and green fluorescent protein (GFP) expression (green channel) indicates transfected cells. For each protocol, LRP4 antibody was visualized with <t>Cy3</t> anti-mouse IgG (red fluorescence). CBA = cell-based assay; LRP4-IgG = lipoprotein receptor-related protein 4; mf-CBA = methanol-fixed CBA; pf-CBA = paraformaldehyde-fixed CBA.
Donkey Cy3 Conjugated Anti Rabbit, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Proteintech fluorescent antibody
Three protocols of CBA were performed: l-CBA (A–C), pf-CBA (D–F), and mf-CBA (G–I). 4′,6-diamidino-2-phenylindole (DAPI) (blue channel) shows nuclear staining, and green fluorescent protein (GFP) expression (green channel) indicates transfected cells. For each protocol, LRP4 antibody was visualized with <t>Cy3</t> anti-mouse IgG (red fluorescence). CBA = cell-based assay; LRP4-IgG = lipoprotein receptor-related protein 4; mf-CBA = methanol-fixed CBA; pf-CBA = paraformaldehyde-fixed CBA.
Fluorescent Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


DPC-Exos transported miR-218-5p from DPCs to HFSCs. (A) Fluorescence imaging of HFSCs after treatment with DiI-labeled DPC-Exos (scale bar = 50 μm). (B) Schematic of the DPC–HFSC co-culture system with fluorescence showing intercellular transfer of exosomal miR-218–5p. (C) MiR-218–5p expression in HFSCs after transfection with Cy3-miR-218–5p (unpaired two-tailed t -test, n = 3). (D) MiR-218–5p expression in HFSCs after DPCs were transfected with siRNA-Drosha (unpaired two-tailed t -test, n = 3). (E) MiR-218–5p expression in HFSCs after DPCs were treated with GW4869 (unpaired two-tailed t -test, n = 3). ∗∗ P < 0.01.

Journal: Non-coding RNA Research

Article Title: Exosomal miRNA-218–5p derived from low-passage dermal papilla cells modulates hair follicle growth and development

doi: 10.1016/j.ncrna.2026.01.004

Figure Lengend Snippet: DPC-Exos transported miR-218-5p from DPCs to HFSCs. (A) Fluorescence imaging of HFSCs after treatment with DiI-labeled DPC-Exos (scale bar = 50 μm). (B) Schematic of the DPC–HFSC co-culture system with fluorescence showing intercellular transfer of exosomal miR-218–5p. (C) MiR-218–5p expression in HFSCs after transfection with Cy3-miR-218–5p (unpaired two-tailed t -test, n = 3). (D) MiR-218–5p expression in HFSCs after DPCs were transfected with siRNA-Drosha (unpaired two-tailed t -test, n = 3). (E) MiR-218–5p expression in HFSCs after DPCs were treated with GW4869 (unpaired two-tailed t -test, n = 3). ∗∗ P < 0.01.

Article Snippet: After three times washing with PBS containing 0.1 % Tween-20 (Solarbio, China, Cat. No. ST825), secondary antibodies Cy3-conjugated Goat Anti-Mouse lgG (Proteintech, China, Cat No. SA00009-1) and Fluorescein (FlTc)-conjugated Goat Anti-Mouse lgG (Proteintech, China, Cat No. SA00003-1) were applied for incubation.

Techniques: Fluorescence, Imaging, Labeling, Co-Culture Assay, Expressing, Transfection, Two Tailed Test

Three protocols of CBA were performed: l-CBA (A–C), pf-CBA (D–F), and mf-CBA (G–I). 4′,6-diamidino-2-phenylindole (DAPI) (blue channel) shows nuclear staining, and green fluorescent protein (GFP) expression (green channel) indicates transfected cells. For each protocol, LRP4 antibody was visualized with Cy3 anti-mouse IgG (red fluorescence). CBA = cell-based assay; LRP4-IgG = lipoprotein receptor-related protein 4; mf-CBA = methanol-fixed CBA; pf-CBA = paraformaldehyde-fixed CBA.

Journal: Neurology® Neuroimmunology & Neuroinflammation

Article Title: Low Prevalence and Inconsistency of LRP4-IgG Detection in Suspected Myasthenia Gravis

doi: 10.1212/NXI.0000000000200554

Figure Lengend Snippet: Three protocols of CBA were performed: l-CBA (A–C), pf-CBA (D–F), and mf-CBA (G–I). 4′,6-diamidino-2-phenylindole (DAPI) (blue channel) shows nuclear staining, and green fluorescent protein (GFP) expression (green channel) indicates transfected cells. For each protocol, LRP4 antibody was visualized with Cy3 anti-mouse IgG (red fluorescence). CBA = cell-based assay; LRP4-IgG = lipoprotein receptor-related protein 4; mf-CBA = methanol-fixed CBA; pf-CBA = paraformaldehyde-fixed CBA.

Article Snippet: For its detection, Cy3 AffiniPure Donkey Anti-Mouse IgG (H + L) (Jackson Immuno Research #715-165-151) was used as the secondary antibody (1:1000).

Techniques: Staining, Expressing, Transfection, Fluorescence, Cell Based Assay